The hepatoprotective and antioxidant activity of Bauhinia hookeri ethanol extract (BHE) against CCl4-induced liver injury was\ninvestigated in mice. BHE was administered (500 and 1000mg/kg/day) along with CCl4 for 6 weeks. The hepatic marker enzymes:\nalanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined in the serum.\nTheantioxidant parameters: glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase\n(GR), glutathione transferase (GST), and malondialdehyde (MDA) were estimated in the liver homogenate. BHE treatment\nsignificantly inhibited the CCl4-induced increase in ALT (44 and 64%), AST (36 and 46%), ALP (28 and 42%), and MDA (39 and\n51%) levels at the tested doses, respectively. Moreover, BHE treatment markedly increased the activity of antioxidant parameters\nGSH, GPx, GR, GST, and SOD. Histological observations confirmed the strong hepatoprotective activity. These results suggest\nthat a dietary supplement of BHE could exert a beneficial effect against oxidative stress and various liver diseases by enhancing\nthe antioxidant defense status, reducing lipid peroxidation, and protecting against the pathological changes of the liver. The\nhepatoprotective activity of BHE is mediated, at least in part, by the antioxidant effect of its constituents. The active constituents of\nBHE were identified by HPLC-PDA-ESI/MS/MS.
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